A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which results in chromosome instability, and therefore could lead to tumorigenesis. This protein was discovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a essential position in promoting mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are explained. Two supplemental splice variants were being claimed but in-depth nucleotide sequence information and facts will not be nevertheless out there.
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Blocking peptides are peptides that bind specifically for the target antibody and block antibody binding. These peptide commonly has the epitope identified because of the antibody.
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This guideline outlines further more possible optimization of mobile and immunoassay parameters to make sure the very best benefits are...
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This guideline outlines additional possible optimization of mobile and immunoassay parameters to guarantee the very best results are...
Antibodies certain to the blocking peptide now not bind towards the epitope on the focus on protein. By comparing the staining with the blocked antibody compared to the antibody by itself, one can see which staining is unique.
Goal Details Cyclin E belongs on the extremely conserved cyclin spouse and children, whose associates are characterised by a spectacular periodicity in protein abundance throughout the mobile cycle. Cyclins perform as regulators of CDK kinases. Unique cyclins show unique expression and degradation styles which contribute to your temporal coordination of every mitotic occasion. Cyclin E varieties a posh with and functions being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and it is degraded as cells progress by S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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